IMR-90 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85020204, human lung (fetal), Fibroblast

• Synonyme(s) : I90 Cells, IMR90 Cells, Institute for Medical Research-90 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lung (fetal)
• Description: Human Caucasian fetal lung fibroblast
• Conditionnement: tube of 5 μg 85020204-DNA-5UG; pkg of vial of cells 85020204-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 46, diploid
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian foetal lung fibroblast

Description de la lignée cellulaire

Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (Sigma catalogue no. 90020107) and MRC-5 cells (Sigma catalogue no. 84101801). The cells have a finite lifespan. Available as a growing order only.

Application

IMR-90 human cell line has been used to study the cytotoxicity of hydroxyapatite/gold/arginine (HAp/Au/arginine) nanoparticles.

Virus studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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