Hep2 (Clone 2B) (HeLa derivative)

85011412, human cervix, Spherical

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: Hep2 (Clone 2B) (HeLa derivative), 85011412
• Source biologique: human cervix
• Conditionnement: tube of 5 μg 85011412-DNA-5UG; pkg of vial of cells 85011412-1VL
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Spherical
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human cervix carcinoma

Description de la lignée cellulaire

This cell line has been adapted for growth in suspension. Cells support growth of arboviruses, measles virus, poliovirus 1, adenovirus 3 and VSV (Indiana strain). This cell line has been found to be indistinguishable from Hela by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Application

Tumourigenicity and virus studies: arboviruses, measles, poliovirus 1, Ad 3 and VSV

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Cells grow in suspension and may form clusters.

Autres remarques

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