ANGM-CSS

8040401, human brain, Fusiform, can form heaps of cells

• Code UNSPSC : 41106514

Propriétés

• product name: ANGM-CSS, 08040401
• Source biologique: human brain
• Mode de croissance: Not specified
• Caryotype: Near tetraploid; range 88 to 91 - see Cell Line Description for more information
• Morphologie: Fusiform, can form heaps of cells
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human glioblastoma

Description de la lignée cellulaire

A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

Milieu de culture

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

Autres remarques

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