VCaP Cell Line human

6020201, human prostate (metastasis), Epithelial

• Synonyme(s) : Human VCaP Cells, VCaP Cell Culture
• Code UNSPSC : 41106514

Propriétés

• product name: VCaP Cell Line human, 06020201, Prostate Cancer Metastasis
• Source biologique: human prostate (metastasis)
• Mode de croissance: Adherent
• Caryotype: Refer to reference (Korenchuk et al., 2001)
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Androgen receptor
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Prostate Cancer Metastasis

Description de la lignée cellulaire

This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19

Milieu de culture

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.