MOCHA

2111901, mouse skin, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: MOCHA, 02111901
• Source biologique: mouse skin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse Skin Fibroblast

Description de la lignée cellulaire

Mocha is a naturally occuring mouse mutant with a null mutation in the delta subunit of AP-3 complex. In Mocha cells, the ß3 amd μ3 subunits coassmeble into a heterodimer whereas the sigma 3 subunit remains monomeric. The mouse has a similar phenotype to the human genetic disorder, Hermansky Pudlak Syndrome (HPS). In both mice and humans, AP-3 deficiency results in hypopigmentation because of abnormalities in melanosomes. The Mocha cell line can be used to investigate the assembly of AP-3 complexes and AP-3 function.

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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