RCP

00031626

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat brain
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Endothelial
• Produits: Not specified
• Récepteurs: Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Choroid Plexus

Description de la lignée cellulaire

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

Application

Toxicology, cellular signalling and pharmacology studies

Milieu de culture

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.1% Epidermal Growth Factor(EGF) (E9644), 1% Insulin–Transferrin–Selenium(ITS) (I3146)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Autres remarques

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