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C7559

Sigma-Aldrich

Chloroforme

≥99%, PCR Reagent, contains amylenes as stabilizer

Synonyme(s) :

Trichlorométhane, Trichlorure de méthylidyne

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About This Item

Formule empirique (notation de Hill):
CHCl3
Numéro CAS:
67-66-3
Poids moléculaire :
119.38
Numéro Beilstein :
1731042
Numéro CE :
200-663-8
Numéro MDL:
MFCD00000826
Code UNSPSC :
12191502
ID de substance PubChem :
24892970
Nomenclature NACRES :
NA.28

Qualité

PCR Reagent

Niveau de qualité

200

Densité de vapeur

4.1 (vs air)

Pression de vapeur

160 mmHg ( 20 °C)

Pureté

≥99%

Forme

liquid

Contient

amylenes as stabilizer

Conditionnement

vial of 1.5 mL

Technique(s)

PCR: suitable

Couleur

colorless

Indice de réfraction

n20/D 1.445 (lit.)

Point d'ébullition

60.5-61.5 °C (lit.)

Pf

−63 °C (lit.)

Densité

1.492 g/mL at 25 °C (lit.)

Activité étrangère

DNase, RNase, none detected

Chaîne SMILES 

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

Clé InChI

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Description générale

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.

Application

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Organes cibles

Central nervous system, Liver,Kidney

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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