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Key Documents

C-23220

Sigma-Aldrich

Fibroblast Growth Medium 2

Basal Medium, 500 ml

Synonyme(s) :

Recommended with C-39325 or C-39320

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

Forme

aqueous solution

Conditionnement

pkg of 500 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Fibroblast Basal Medium 2 (supplements sold separately)

To form a complete medium, it should be combined with the media supplements: C-39325 or C-39320

Application

Fibroblast Growth Medium 2 is a low-serum medium and has been developed for the in vitro cultivation of adult Human Fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), adult donorHuman Pulmonary Fibroblasts (HPF)Human Aortic Adventitial Fibroblasts (HAoAF)Human Uterine Fibroblasts (HUF)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Fibroblast Growth Medium 2 are subjected to comprehensive quality control tests using primary human adult fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested adult fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Stockage et stabilité

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Procédure de repiquage

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Articles

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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