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Key Documents

C-12206

Sigma-Aldrich

Human Umbilical Vein Endothelial Cells 2 (HUVEC 2)

Single donor, isolated in Growth Medium 2, 500,000 cryopreserved cells

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Forme

aqueous solution

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Température de stockage

−196°C

Application

Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord. They are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. HUVEC are isolated from single donors in Endothelial Cell Growth Medium 2, which is free of ECGS and contains additional IGF and VEGF, and provided in a cryopreserved format. (Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.)

Qualité

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Avertissement

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Procédure de repiquage

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Autres remarques

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P1Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Produits recommandés

Recommended Primary Cell Culture Media:Link

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Protocoles

The Endothelial Cell Transwell Migration and Invasion Assay

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

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