C0620

CDK4/Cyclin D1, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : BCL1, CMM3, D11S2878E, MG Cl4458, PRAD1, PSK-J3, U21 B31
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Niveau de qualité: 200
• Gamme de produits: PRECISIO^® Kinase
• Pureté: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 12-16 nmol/min·mg
• Poids mol.: CDK4 subunit ~57 kDa; cyclin D1 ~61 kDa
• Numéro d'accès UniProt: P11802; P24385
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CCND1(595) , CDK4(1019)

Description

Actions biochimiques/physiologiques

CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex.CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.

Forme physique

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)