C0543

Rat Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

• Synonyme(s) : Type IV collagen, EHS-collagen
• Numéro CAS: 9007-34-5
• Numéro CE : 232-697-4
• Numéro MDL: MFCD00130825
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.75

Propriétés

• product name: Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
• Source biologique: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Niveau de qualité: 200
• Type: Type IV (Miller)
• Gamme de produits: BioReagent
• Forme: lyophilized powder
• Conditionnement: glass bottle of 1 vial
• Technique(s): cell culture | mammalian: suitable
• Couverture de surface: 6‑10 μg/cm²
• Solubilité: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• Numéro d'accès UniProt: B1AVK5; P02463; P08122; Q63ZW6; Q9QZS0
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Col4a1(12826) , Col4a2(12827) , Col4a3(12828) , Col4a5(12830) , Col4a6(94216)

Description

Description générale

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.
• in hydrogel fabrication.
• to coat cell culture plates for cell differentiation.
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Actions biochimiques/physiologiques

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Autres remarques

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Quantité

0.75 mg/vial

Notes préparatoires

Chloroform treated.
Pepsin is not used in the preparation.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)