B3640
Anti-Biotine
affinity isolated antibody, lyophilized powder
Synonyme(s) :
Anti-Biotin, Anti Biotin Antibody, Anti Biotin Antibody - Anti-Biotin antibody produced in goat, Anti-Biotin Antibody
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
goat
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
lyophilized powder
Technique(s)
immunoelectrophoresis: suitable
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
La spécificité et la réactivité sont déterminées par la double diffusion d'Ouchterlony et l'immunoélectrophorèse par rapport au complexe biotine-protéine porteuse. Aucune réactivité n'est observée par rapport à la protéine porteuse.
Biotin is considered as an essential nutrient, though it is know to be secreted by some of the intestinal bacteria. It is found in liver and brain tissues in minute quantities.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.
Immunogène
Conjugué biotine-KLH
Application
Dans certaines applications, la localisation de sondes biotinylées avec de l'avidine produit des niveaux de bruit de fond élevés. Des réactifs anti-biotine peuvent être substitués à l'avidine pour diminuer la liaison non spécifique.
Anti-Biotin antibody produced in goat has been used in multi-well plate assay, to identify desthiobiotinylating (analog of biotin) proteins. It has also been used in Colourimetric enzyme immunoassay.
Actions biochimiques/physiologiques
Biotin serves as a cofactor for carboxylases, necessary for oxidative metabolism. Biotin absorption in small intestine is reduced on consumption of avidin rich food such as egg white. Thus, greatly decreasing its bioavailability, leading to egg white injury. Reduced levels of biotin is normally observed during pregnancy. Biotin deficiency might be congenital, with defective proteins involved in biotin homeostasis.
Forme physique
Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2
Reconstitution
Reconstitute with 0.135 M sodium chloride.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
Marcus Braun et al.
Nature cell biology, 13(10), 1259-1264 (2011-09-06)
Short regions of overlap between ends of antiparallel microtubules are central elements within bipolar microtubule arrays. Although their formation requires motors, recent in vitro studies demonstrated that stable overlaps cannot be generated by molecular motors alone. Motors either slide microtubules
Zdenek Lansky et al.
Cell, 160(6), 1159-1168 (2015-03-10)
Cytoskeletal remodeling is essential to eukaryotic cell division and morphogenesis. The mechanical forces driving the restructuring are attributed to the action of molecular motors and the dynamics of cytoskeletal filaments, which both consume chemical energy. By contrast, non-enzymatic filament crosslinkers
A R Prescott et al.
Journal of cell science, 102 ( Pt 1), 103-112 (1992-05-01)
The turnover of microtubules was studied in the processes of PtK2 cells, after treatment with the cytokine scatter factor (SF), using micro-injected biotin-tubulin as a reporter of new microtubule growth. Cells treated with SF became dispersed and fibroblast-like in morphology
Avidin-Biotin Interactions: Methods and Applications, 111-111 (2008)
Nutritional Sciences: From Fundamentals to Food, 437-437 (2012)
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