A8849

α₂-Antiplasmin from human plasma

lyophilized powder, ≥5 units/mg protein

• Numéro CAS: 138757-15-0
• Numéro MDL: MFCD00130471
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human plasma
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥5 units/mg protein
• Conditionnement: vial of ≥0.5 mg (Protein based on E1%/280 = 6.7)
• Numéro d'accès UniProt: P08697
• Température de stockage: −20°C
• Informations sur le gène: human ... SERPINF2(5345)

Description

Description générale

α2-Antiplasmin (α2AP) is a glycoprotein and a member of the serine protease inhibitor (serpin) family. The protein is mainly synthesized in the liver and found in the kidney and brain. The serpin peptidase inhibitor, clade F member 2 (SERPINF2) gene is mapped to human chromosome 17p13.3.

Application

α2-Antiplasmin from human plasma has been used as a protease inhibitor to treat human microvascular endothelial cells (HMEC-1) and demonstrate the role of factors responsible for endothelial cells detachment. It has also been used to study its effect on the release of proteolytic activity under reducing conditions in therapeutic human serum albumin-containing products with a new neoepitope endopeptidase immunoassay.

Actions biochimiques/physiologiques

α2-Antiplasmin (α2AP) is an antifibrinolytic protein and an important inhibitor of plasmin. α2AP mediates clot formation by covalently cross-linking with the glutamine and the lysine residue of fibrin clot via activated factor XIII (FXIIIa). This prevents the clot from extreme degradation by plasmin. An inborn insufficiency of α2AP results in an autosomal recessive condition leading to a rare bleeding disorder. The severity of the bleeding condition is defined by the individual′s genetic variation. Studies show lower levels of α2AP associated with disseminated intravascular coagulation (DIC) and thrombolytic therapy. Elevated levels of α2AP have been reported in a patient with ischemic stroke.

Définition de l'unité

One unit will reduce the activity of two units of plasmin by 50% at 37 °C, pH 7.2, using Val-Leu-Lys-p-nitroanilide.

Forme physique

Lyophilized powder containing Bis-tris buffer and NaCl.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315 - H319 - H335
• Conseils de prudence: P261 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves