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Key Documents

A1009

Sigma-Aldrich

Albumin methylated from bovine serum

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About This Item

Numéro MDL:
MFCD00145745
Code UNSPSC :
12352202
eCl@ss :
42010201
Nomenclature NACRES :
NA.26

Source biologique

bovine

Niveau de qualité

200

Forme

powder

Origine

USA origin

Technique(s)

affinity chromatography: suitable
fractionation: suitable

Impuretés

≤10% water (Karl Fischer)

Numéro d'accès UniProt

P02769

Température de stockage

2-8°C

Informations sur le gène

bovine ... ALB(280717)

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Application

Albumin methylated from bovine serum (mBSA) has been used as a coating for immunoplates in enzyme-linked immunosorbent assay (ELISA) of anti-dsDNA IgG antibodies. It has also been used to induce arthritis in mice models.
As an affinity ligand on diatomaceous earth support (MAK chromatography), a medium for fractionation of different forms of DNA and for separating aminoacyl-tRNAs. Different modes of binding of double-stranded, denatured, and replicating forms of DNA account for the efficacy of the method.

Actions biochimiques/physiologiques

Albumin is a major circulating component of the plasma and is a highly soluble multifunctional protein. It plays a role in several physiological and pharmacological processes. Albumin binds to metals, cholesterol, hormones, bile pigments, drugs, and fatty acids to facilitate their transport. It regulates osmotic pressure and distributes fluids between various compartments.

Notes préparatoires

Methyl ester of albumin prepared per Mandell, J.D. and Hershey, A.D., Anal. Biochem., 1, 66 (1960).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Albumine de sérum bovin chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

Sigma-Aldrich

A1933

Albumine de sérum bovin

Albumine de sérum bovin lyophilized powder, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A2153

Albumine de sérum bovin

Fractionation of nucleic acids with the methylated albumin column.
N SUEOKA et al.
Journal of molecular biology, 4, 161-172 (1962-03-01)
T Hofstaetter et al.
Biochimica et biophysica acta, 518(1), 53-60 (1978-03-29)
The binding of methylated albumin to DNA, the basis of the chromatography on columns of kieselguhr coated with methylated albumin (MAK chromatography), was investigated. Scatchard plots revealed only one mode of interaction with fully double-stranded DNA. The complexes should be
Kasper Almholt et al.
Journal of immunology (Baltimore, Md. : 1950), 200(3), 957-965 (2017-12-29)
Genetic absence of the urokinase-type plasminogen activator (uPA) reduces arthritis progression in the collagen-induced arthritis (CIA) mouse model to an extent just shy of disease abrogation, but this remarkable observation has not been translated into therapeutic intervention. Our aim was
Fractionation of amino acyl-acceptor RNA on a methylated albumin column.
N SUEOKA et al.
Proceedings of the National Academy of Sciences of the United States of America, 48, 1454-1461 (1962-08-01)
Mohammad Haque et al.
Scientific reports, 6, 20588-20588 (2016-02-06)
Pluripotent stem cells (PSCs) have the potential to produce almost all of the cells in the body, including regulatory T cells (Tregs). However, the exact conditions required for the development of antigen (Ag)-specific Tregs from PSCs (i.e., PSC-Tregs) are not
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