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Key Documents

A0410

Sigma-Aldrich

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

Synonyme(s) :

5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP

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About This Item

Formule empirique (notation de Hill):
C12H20N3O14P3 · xNa+
Numéro CAS:
936327-10-5
Poids moléculaire :
523.22 (free acid basis)
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Pureté

≥85%

Forme

lyophilized powder

Conditions d'expédition

dry ice

Température de stockage

−70°C

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Application

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
  • to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
  • for the generation of chromatin immunoprecipitation (ChIP) probes
  • in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Actions biochimiques/physiologiques

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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