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Sigma-Aldrich

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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About This Item

Numéro MDL:
MFCD05865408
Code UNSPSC :
12352108
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Pureté

≥90% (HPCE)

Forme

powder

Fabricant/nom de marque

ATTO-TEC GmbH

Transmittance

254 nm
647 nm

Fluorescence

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorption UV

λ: 645-651 nm Amax

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Description générale

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Cytokinesis,Volume 137 of Methods in Cell Biology (2017)
Jean-Manuel Segura et al.
The Journal of biological chemistry, 283(35), 24254-24263 (2008-06-27)
CD8(+) cytotoxic T lymphocytes (CTL) can recognize and kill target cells expressing only a few cognate major histocompatibility complex (MHC) I-peptide complexes. This high sensitivity requires efficient scanning of a vast number of highly diverse MHC I-peptide complexes by the
Daniel Puntener et al.
Journal of virology, 85(1), 481-496 (2010-11-05)
Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid.
Duncan Hill et al.
Biomedical microdevices, 13(4), 759-767 (2011-05-12)
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a
Laura Pastorino et al.
Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)
The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their
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