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71386

Sigma-Aldrich

Chlorure de sodium solution

BioUltra, for molecular biology, ~5 M in H2O

Synonyme(s) :

Halite, NaCl, sodium chloride

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About This Item

Formule linéaire :
NaCl
Numéro CAS:
Poids moléculaire :
58.44
Numéro Beilstein :
3534976
Numéro MDL:
Code UNSPSC :
12352302
ID de substance PubChem :
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

Gamme de produits

BioUltra

Forme

liquid

Concentration

~5 M in H2O

Technique(s)

DNA extraction: suitable
LAMP: suitable

Impuretés

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

Indice de réfraction

n20/D 1.378

pH

5.0-7.0 (25 °C)

Solubilité

water: soluble

Densité

1.186 g/mL (5 M in water)

Traces de cations

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorption UV

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

Adéquation

suitable for molecular biology

Application(s)

genomic analysis

Chaîne SMILES 

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

Clé InChI

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Description générale

Sodium chloride is a vital nutrient that aids normal body functions in humans and animals. This chemical is commonly found in nature with a wide variety of biochemical applications, like intravenous fluids and density gradients. It is also used as a diluent to increase ionic strength in buffers or culture media. Sodium chloride is crucial for maintaining plasma osmolality and the extracellular fluid volume of the body. Excess NaCl leads to increased blood pressure, which has implications for cardiovascular and renal diseases. Sterile NaCl is a component of eye drop solutions. Traditionally, it has been used in high concentrations to preserve food since most bacteria cannot grow in high-salt conditions.

Application

Sodium chloride solution has been used as a component of:
  • polyethylene glycol (PEG) buffer for the purification of double-tagged products
  • NT2 buffer for the preparation of antibody-coated Protein A Sepharose (PAS) beads and immunoprecipitation of ribonucleoproteins (RNPs)
  • chilled lysis buffer for the isolation of single nuclei from mice kidney

Caractéristiques et avantages

  • Geologically stable
  • Suitable for purification, precipitation, and crystallization

Autres remarques

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Francis Kim et al.
JAMA, 311(1), 45-52 (2013-11-19)
Hospital cooling improves outcome after cardiac arrest, but prehospital cooling immediately after return of spontaneous circulation may result in better outcomes. To determine whether prehospital cooling improves outcomes after resuscitation from cardiac arrest in patients with ventricular fibrillation (VF) and
Laurent Dortet et al.
Journal of clinical microbiology, 52(7), 2359-2364 (2014-04-25)
Multidrug-resistant Acinetobacter baumannii isolates, particularly those that produce carbapenemases, are increasingly reported worldwide. The biochemically based Carba NP test, extensively validated for the detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified to detect carbapenemase production in
Alexander W Wyatt et al.
Genome biology, 15(8), 426-426 (2014-08-27)
Genomic analyses of hundreds of prostate tumors have defined a diverse landscape of mutations and genome rearrangements, but the transcriptomic effect of this complexity is less well understood, particularly at the individual tumor level. We selected a cohort of 25
I Dzneladze et al.
Leukemia, 29(7), 1485-1495 (2015-03-05)
In this study, we investigated the role of inositol polyphosphate-4-phosphatase, type-II (INPP4B) in acute myeloid leukemia (AML). We observed that AML patients with high levels of INPP4B (INPP4B(high)) had poor response to induction therapy, shorter event-free survival and shorter overall
David G Guzzardi et al.
Journal of the American College of Cardiology, 66(8), 892-900 (2015-08-22)
Suspected genetic causes for extracellular matrix (ECM) dysregulation in the ascending aorta in patients with bicuspid aortic valves (BAV) have influenced strategies and thresholds for surgical resection of BAV aortopathy. Using 4-dimensional (4D) flow cardiac magnetic resonance imaging (CMR), we

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