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58030

Sigma-Aldrich

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonyme(s) :

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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About This Item

Formule empirique (notation de Hill):
C19H13ClIN5O2
Numéro CAS:
146-68-9
Poids moléculaire :
505.70
Numéro Beilstein :
4093224
Numéro CE :
205-676-2
Numéro MDL:
MFCD00011961
Code UNSPSC :
12352102
ID de substance PubChem :
57651636
Nomenclature NACRES :
NA.25

Gamme de produits

BioReagent

Niveau de qualité

200

Pureté

≥97.0% (calc. on dry substance, NT)

Pf

240 °C (dec.) (lit.)
~245 °C (dec.)

Solubilité

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

Application(s)

histology

Chaîne SMILES 

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

Clé InChI

JORABGDXCIBAFL-UHFFFAOYSA-M

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Application

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Electron acceptor for the colorimetric assay of various dehydrogenases

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H228,H335

Classification des risques

Flam. Sol. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

4.1B - Flammable solid hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Christopher Auger et al.
Electrophoresis, 33(7), 1095-1101 (2012-04-28)
Phosphoenolpyruvate (PEP)-dependent kinases are central to numerous metabolic processes and mediate the production of adenosine triphosphate (ATP) by substrate-level phosphorylation (SLP). While pyruvate kinase (PK, EC: 2.7.1.40), the final enzyme of the glycolytic pathway is critical in the anaerobic synthesis
Matthildi Valianou et al.
Scientific reports, 9(1), 3015-3015 (2019-03-01)
Tuberous Sclerosis Complex (TSC) and Lymphangioleiomyomatosis (LAM) are caused by inactivating mutations in TSC1 or TSC2, leading to mTORC1 hyperactivation. The mTORC1 inhibitors rapamycin and analogs (rapalogs) are approved for treating of TSC and LAM. Due to their cytostatic and
Antoine Picciocchi et al.
The Journal of biological chemistry, 286(32), 28357-28369 (2011-06-11)
Flavocytochrome b(558) (cytb) of phagocytes is a heterodimeric integral membrane protein composed of two subunits, p22(phox) and gp91(phox). The latter subunit, also known as Nox2, has a cytosolic C-terminal "dehydrogenase domain" containing FAD/NADPH-binding sites. The N-terminal half of Nox2 contains
Yang Hongwei et al.
Ecotoxicology and environmental safety, 59(2), 249-255 (2004-08-26)
Anaerobic biodegradability of organic compounds can be assessed from the changes in organic compounds, the end products, and the activity of microorganisms. In this study, all three of these variables were considered to integrate assessment of the anaerobic biodegradability of
Sergio Ravasio et al.
Biochemistry, 41(25), 8120-8133 (2002-06-19)
The properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803 were determined by means of kinetic and spectroscopic approaches in comparison to those exhibited by the bacterial NADPH-dependent enzyme form. The ferredoxin-dependent enzyme was found to be similar to
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