3447-020-01
Cultrex 3-D Culture Matrix™ Rat Collagen I
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Source biologique
rat
Stérilité
sterile
Poids mol.
300 kDa
Concentration
5 mg/mL
Technique(s)
cell culture | mammalian: suitable
Solubilité
water: miscible
Conditions d'expédition
ambient
Température de stockage
2-8°C
Description générale
The 3-D Culture Matrix™ Rat Collagen I may be used as a gel on which to grow cells or a medium additive, alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. Type I Collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. The 5 mg/ml concentration is very viscous for researchers wanting a thicker Collagen.
Forme physique
Solution in 20 mM Acetic Acid, pH 3.4 - 3.6
Autres remarques
Functional Assay: 3-D Culture: Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH and promotes attachment and growth of murine endothelial SVEC4-10 cells. Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.
Sterility Testing: No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and product stability: Product is stable for a minimum of 3 months from date of shipment if stored at 4°C. Do Not Freeze.
Informations légales
3-D Culture Matrix is a trademark of Trevigen, Inc.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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