3023
AptaFluor® SAH Methyltransferase Assay
Synonyme(s) :
Methyltransferase Activity Assay
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41122100
Nomenclature NACRES :
NA.84
Produits recommandés
Conditions d'expédition
dry ice
Température de stockage
−70°C
Description générale
The AptaFluor® SAH Methyltransferase TR-FRET Assay is a universal assay for enzymes that convert S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH), with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay can be used for all enzymes within the histone methyltransferase (HMT) and DNA methyltransferase (DNMT) families. The assay is designed specifically for high-throughput screening (HTS), with a simple mix-and-read format and 2 liquid addition steps. It is run in an endpoint mode: methyltransferase (MT) enzyme reactions are quenched with a universal stop reagent (provided) and then the detection reagents are added.
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
View full Transcreener® product list
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
- Simple, mix-and-read format ideal for HTS.
- Robust MT detection under initial velocity conditions (=20% conversion of SAM to SAH).
- Accommodates SAM concentrations as low as 100 nM and as high as 5 μM.
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
View full Transcreener® product list
Quantité
3023-B = 100 assay, 96-well
3023-1K = 1,000 assay, 384-well
3023-1K = 1,000 assay, 384-well
Forme physique
Kit with buffered aqueous solutions
Informations légales
AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
L Latini et al.
Cell death & disease, 5, e1404-e1404 (2014-09-05)
Spinal cord injury (SCI) is a devastating condition of CNS that often results in severe functional impairments for which there are no restorative therapies. As in other CNS injuries, in addition to the effects that are related to the primary
Kalyan C Kondapalli et al.
The Journal of biological chemistry, 287(43), 36239-36250 (2012-09-06)
Human NHA2, a newly discovered cation proton antiporter, is implicated in essential hypertension by gene linkage analysis. We show that NHA2 mediates phloretin-sensitive Na(+)-Li(+) counter-transport (SLC) activity, an established marker for hypertension. In contrast to bacteria and fungi where H(+)
Maria Teresa Viscomi et al.
Autophagy, 8(2), 222-235 (2012-01-18)
Autophagy is the evolutionarily conserved degradation and recycling of cellular constituents. In mammals, autophagy is implicated in the pathogenesis of many neurodegenerative diseases. However, its involvement in acute brain damage is unknown. This study addresses the function of autophagy in
Brian G Coon et al.
The Journal of cell biology, 208(7), 975-986 (2015-03-25)
Endothelial responses to fluid shear stress are essential for vascular development and physiology, and determine the formation of atherosclerotic plaques at regions of disturbed flow. Previous work identified VE-cadherin as an essential component, along with PECAM-1 and VEGFR2, of a
Mirja Tamara Prentzell et al.
Cell, 184(3), 655-674 (2021-01-27)
Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 and G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface of lysosomes. They act in a non-redundant manner to anchor
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique