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Key Documents

3020

Sigma-Aldrich

Transcreener® AMP2/GMP2 TR-FRET Assay

Synonyme(s) :

TR-FRET assay, Transcreener assay

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About This Item

Code UNSPSC :
41122100
Nomenclature NACRES :
NA.84

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

The Transcreener® AMP2/GMP2 TR-FRET Red Assay is a competitive immunoassay for enzymes that produce AMP or GMP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay relies on a highly specific monoclonal antibody that recognizes AMP or GMP with more than 1,000-fold selectivity over substrate nucleotides, including ATP, cAMP, or cGMP. Enzymes that can be used with the assay include ubiquitin, SUMO, nucleic acid and protein ligases, phosphodiesterases (PDEs), and synthetases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® AMP2/GMP2 TR-FRET Red Assay provides the following benefits:
  • Accommodates substrate concentrations ranging from 0.1 μM to 1,000 μM.
  • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
  • The only assay method that allows detection of unmodified AMP and GMP without using coupling enzymes.
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

Quantité

3020-1K = 1,000 assay, 384-well
3020-10K = 10,000 assay, 384-well

Forme physique

Kit with buffered aqueous solutions

Informations légales

Transcreener is a registered trademark of BellBrook Labs

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M J Abramowicz et al.
The Journal of clinical investigation, 99(12), 3018-3024 (1997-06-15)
Thyroid gland agenesis is the most common cause of congenital hypothyroidism and is usually sporadic. We investigated a brother and sister from consanguineous parents, ascertained through systematic newborn screening, and initially diagnosed with thyroid agenesis. Careful cervical ultrasonography in both

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