G6257

Glutaraldéhyde solution

Grade II, 25% in H₂O

• Synonyme(s) : Dialdéhyde glutarique solution, Pentane-1,5-dial
• Formule linéaire : OHC(CH₂)₃CHO
• Numéro CAS: 111-30-8
• Poids moléculaire : 100.12
• Numéro Beilstein : 605390
• Numéro MDL: MFCD00007025
• Code UNSPSC : 12352114
• ID de substance PubChem : 24895250
• Nomenclature NACRES : NA.21

Propriétés

• Type: Grade II
• Niveau de qualité: 200
• Forme: liquid
• Concentration: 25% in H₂O
• Technique(s): electron microscopy: suitable
• Couleur: colorless
• Plage de pH utile: 2.9
• Pf: -10 °C ((14 °F ))
• Solubilité: water: soluble
• Application(s): cell analysis; sample preparation
• Chaîne SMILES: [H]C(CCCC([H])=O)=O
• InChI: 1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2
• Clé InChI: SXRSQZLOMIGNAQ-UHFFFAOYSA-N

Description

Description générale

Glutaraldehyde, known as Glutaric dialdehyde or Pentane-1,5-dial, serves as a versatile reagent with applications spanning various research domains, including cell biology and biochemical research. It finds applications as a protein crosslinker, aids in enzyme immobilization for microscopy, and contributes to histochemistry and cytochemistry processes. As an amine-reactive homobifunctional crosslinker, it acts as a cell fixative before procedures like SDS-PAGE, staining, or electron microscopy, making it a valuable tool for diverse morphological studies.

Glutaraldehyde demonstrates efficacy in crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it allows direct coupling of biotin hydrazides to nucleic acids, offering potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. Moreover, in histology studies, Glutaraldehyde is employed for preserving tissue sections and preparing them for detailed examination.

Application

Glutaraldehyde solution has been used:

• for fixation of mouse and esophageal cancer cell lines
• to crosslink amyloid-β protein
• to crosslink gelatin in 3D scaffold preparation of trachea

Actions biochimiques/physiologiques

Glutaraldehyde is an effective protein crosslinker and finds application in techniques like enzyme immobilisation microscopy, histochemistry and cytochemistry. It exists in different forms under acidic or neutral conditions. It is toxic to aquatic organisms. Its allergic nature leads to hypersensitivity reactions. Contact of glutaraldehyde vapors in endoscopy contributes to Colitis.

Caractéristiques et avantages

• Versatile and adaptable for wide variety of research applications
• Ready-made solution reduces the need for preparation time

Conditionnement

Not refrigerated en route

Attention

This grade is not recommended for use as an electron microscopy fixative.

Notes préparatoires

Not specially purified.

Autres remarques

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To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Clause de non-responsabilité

“The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.”

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07,GHS08,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H332 - H314 - H317 - H334 - H335 - H410
• Conseils de prudence: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Risques supp: EUH071
• Code de la classe de stockage: 8A - Combustible, corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter