D1054

Dihydrorhodamine 123

≥95% purity, powder

• Synonyme(s) : DHR 123; Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate; Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester
• Formule empirique (notation de Hill): C₂₁H₁₈N₂O₃
• Numéro CAS: 109244-58-8
• Poids moléculaire : 346.38
• Beilstein: 5989965
• Numéro MDL: MFCD00285712
• Code UNSPSC : 12171500
• ID de substance PubChem : 24893337
• Nomenclature NACRES : NA.47

Propriétés

• Nom du produit: Dihydrorhodamine 123, ≥95%
• Niveau de qualité: 200
• Essai: ≥95%
• Forme: powder
• Technique(s): titration: suitable
• Couleur: pink
• Solubilité: DMSO: >5 mg/mL
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: COC(=O)c1ccccc1C2c3ccc(N)cc3Oc4cc(N)ccc24
• InChI: 1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
• Clé InChI: FNEZBBILNYNQGC-UHFFFAOYSA-N

Description

Description générale

Dihydrorhodamine 123 (DHR123) is an uncharged non-fluorescent dye and a derivative of rhodamine 123 (R123). Dihydrorhodamine 123 (DHR123) is oxidized to fluorescent R123 within cells in the presence of reactive oxygen species and it localizes in mitochondria. It is an analog of 2,7-Dichlorodihydrofluorescein (DCDHF), lipophilic and diffuses through cell membrane.

Application

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

Actions biochimiques/physiologiques

Dihydrorhodamine 123 detects superoxide ions at the single-cell level and is used for probing neutrophils superoxide generation in a respiratory burst event. It is also used for measuring oxidative stress in macrophages and human spermatozoa.

Dihydrorhodamine 123 is used for the detection of ROS (reactive oxygen species). Presence of ROS oxidizes dihydrorhodamine 123 to the fluorescent derivative rhodamine 123. The reaction happens in the presence of a peroxidase or a similar catalyst.

Substrats

Peroxidase substrate

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)