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63011

Millipore

M9 Broth

suitable for microbiology, NutriSelect® Basic

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About This Item

Code UNSPSC :
41171607
Nomenclature NACRES :
NA.74

Stérilité

non-sterile

Niveau de qualité

100

Forme

powder

Composition

ammonium chloride, 1 g/L
disodium hydrogen phosphate, 6 g/L
potassium dihydrogen phosphate, 3 g/L
sodium chloride, 0.5 g/L

Fabricant/nom de marque

NutriSelect® Basic

Technique(s)

microbiological culture: suitable

pH final

7.4±0.2 (25 °C)

Application(s)

food and beverages
life science and biopharma
microbiology

Adéquation

nonselective for Escherichia coli
nonselective for coliforms

Application

M9 Broth is used as a microbiological growth media and for the preparation of phage and plasmid DNA using E. coli K12 strains, acc. to Miller (1987).

Notes préparatoires

Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140)

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificats d'analyse (COA)

Lot/Batch Number
BCCG8471
BCCD5614
BCBX0939
BCBV2133
BCBR0049V

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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ISOGRO®-15N Powder -Growth Medium 98 atom % 15N

Sigma-Aldrich

606871

ISOGRO®-15N Powder -Growth Medium

Magnesium sulfate solution for molecular biology, 1.00 M±0.04 M

Sigma-Aldrich

M3409

Magnesium sulfate solution

Khaled M Khleifat et al.
Current microbiology, 57(4), 364-370 (2008-07-30)
A successful attempt was made to isolate linear alkylbenzene sulfonate (LAS)-degrading bacteria from soil irrigated with wastewater. The isolated bacteria were able to use LAS as sole carbon and energy source. Maximum growth rates on LAS reached only 0.27 h(-1).
D Pérez-Conesa et al.
Journal of food protection, 74(1), 55-62 (2011-01-12)
Carvacrol and eugenol were encapsulated in micellar nonionic surfactant solutions to increase active component concentrations in the aqueous phase and used to treat two strains of Listeria monocytogenes (Scott A and 101) and two strains of Escherichia coli O157:H7 (4388
Pyoung Il Kim et al.
Journal of microbiology and biotechnology, 20(1), 138-145 (2010-02-06)
A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30degreesC. Biosurfactant lipopeptides produced by Bacillus
Nancy Valentine et al.
Applied and environmental microbiology, 71(1), 58-64 (2005-01-11)
Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive
Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage.
H Miller
Methods in enzymology, 152, 145-170 (1987-01-01)

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