DUTP-RO

dUTP

PCR Grade, sodium salt

• Synonyme(s) : 2′-Deoxyuridine 5′-triphosphate sodium salt solution, dUTP sodium salt
• Formule empirique (notation de Hill): C₉H₁₅N₂O₁₄P₃ · xNa⁺
• Numéro CAS: 94736-09-1
• Poids moléculaire : 468.14 (free acid basis)
• Numéro MDL: MFCD01630759
• Code UNSPSC : 41106305
• ID de substance PubChem : 329798931
• Nomenclature NACRES : NA.21

Propriétés

• Niveau de qualité: 100
• Pureté: 99% (dNTP)
• Forme: solution
• Utilisation: sufficient for 125,000 standard reactions (03732720001); sufficient for 31,250 standard reactions (11969056001); sufficient for 6,250 standard reactions (11934554001)
• Conditionnement: pkg of 1,250 μL (11969056001 [100 mM]); pkg of 4 × 1,250 μL (03732720001 [4x100 mM]); pkg of 250 μL (11934554001 [100 mM])
• Fabricant/nom de marque: Roche
• Concentration: <0.9% (dNDP); 100 mM
• Température de stockage: −20°C
• Chaîne SMILES: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O14P3.Na.H/c12-5-3-8(11-2-1-7(13)10-9(11)14)23-6(5)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,12H,3-4H2,(H,18,19)(H,20,21)(H,10,13,14)(H2,15,16,17);;
• Clé InChI: FVFWWPFKJNTHIQ-UHFFFAOYSA-N

Description

Description générale

dUTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Application

dUTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Avoid carryover contamination between PCRs to eliminate a source of false positives.

Qualité

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Forme physique

solution of dUTP water (pH 8.3).

Notes préparatoires

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (30 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 30 μl dUTP to 40 μl Water,PCR grade).

Autres remarques

For general laboratory use.

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash