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CELLPRO-RO

Roche

Réactif de prolifération cellulaire WST-1

suitable for protein quantification, suitable for cell analysis, detection, solution

Synonyme(s) :

Réactif de prolifération cellulaire, wst-1

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.21

Forme

solution

Niveau de qualité

100

Utilisation

sufficient for ≤2,500 tests (11644807001)
 sufficient for ≤800 tests (05015944001)

Conditionnement

bottle of 25 mL (11644807001)
bottle of 8 mL (05015944001)

Fabricant/nom de marque

Roche

Conditions de stockage

protect from light

Technique(s)

protein quantification: suitable

pH optimal

8.0(for physiological conditions our product is buffered pH 7.3)

λmax

440-480 nm

Application(s)

cell analysis
detection

Méthode de détection

colorimetric

Température de stockage

−20°C

Description générale

Sommaire
Ready-to-use solution, containing WST-1 and an electron coupling reagent.
Colorimetric assay (WST-1 based) for the nonradioactive quantification of cell proliferation, cell viability, and cytotoxicity.
La prolifération cellulaire nécessite la réplication de l'ADN génomique. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. ′ The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.

Application

The Cell Proliferation Reagent WST-1 is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
  • Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
  • Assessment of growth inhibitory antibodies and physiological mediators

Caractéristiques et avantages

  • Pratique : Benefit from a ready-to-use reagent.
  • Eliminate radioactive isotopes, washing steps, and additional reagents.
  • Précis : The absorbance obtained strongly correlates to the cell number.
  • Sensible Detect low cell numbers.
  • Rapides : Process a large number of samples using a multi-well ELISA reader.

Principe

The stable tetrazolium salt WST-1 is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.

Notes préparatoires

Concentration de travail : μμ
μ
μμ

When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations.
However please note that the solution may become viscous.


Autres remarques

Pour la recherche en sciences de la vie uniquement. Ne pas utiliser dans des procédures de diagnostic.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Wilfried Weber et al.
Nucleic acids research, 35(17), e116-e116 (2007-09-11)
Although adjustable transgene expression systems are considered essential for future therapeutic and biopharmaceutical manufacturing applications, the currently available transcription control modalities all require side-effect-prone inducers such as immunosupressants, hormones and antibiotics for fine-tuning. We have designed a novel mammalian transcription-control
Johanna Tukler Henriksson et al.
Investigative ophthalmology & visual science, 56(8), 4186-4197 (2015-07-02)
To investigate the effects of IL-13 on goblet cell proliferation, differentiation, and expression of mucin and immunomodulatory genes. Explants were excised from the conjunctiva of young C57BL/6 mice. Cultures received 200 μL per week of either Keratinocyte media (KSFM) or
Deniz M Özata et al.
Endocrine-related cancer, 18(6), 643-655 (2011-08-24)
Adrenocortical carcinoma (ACC) is an aggressive tumor showing frequent metastatic spread and poor survival. Although recent genome-wide studies of ACC have contributed to our understanding of the disease, major challenges remain for both diagnostic and prognostic assessments. The aim of
Rainer Gosert et al.
Antimicrobial agents and chemotherapy, 55(5), 2129-2136 (2011-03-16)
Polyomavirus JC (JCV) replication causes progressive multifocal leukoencephalopathy (PML), a frequently fatal brain disease in immunodeficient patients, yet antiviral drugs are lacking. We characterized the lipid conjugate 1-O-hexadecyloxypropyl-cidofovir (CMX001) regarding JCV (Mad-4) replication in human brain progenitor-derived astrocytes (PDA) and
Chu Zhang et al.
The Prostate, 71(2), 157-167 (2010-07-29)
Preferential bony metastasis of human prostate cancer (PCa) cells contributes to disease mortality and morbidity. Local factors in bone stromal extracellular matrix microenvironment affect tumor growth through paracrine interactions between tumor and stromal cells. Using co-culture and medium transfer, we

Articles

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Protocoles

Protocol Guide: WST-1 Assay for Cell Proliferation and Viability

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

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