BLNI-RO
Roche
Bln I (Avr II)
from Brevibacterium linens
About This Item
Produits recommandés
Source biologique
bacterial (Brevibacterium linens)
Niveau de qualité
Forme
solution
Activité spécifique
10000 U/mL
Conditionnement
pkg of 1,000 U (11558170001 [10 U/μl])
pkg of 200 U (11558161001 [10 U/μl])
Fabricant/nom de marque
Roche
Paramètres
37 °C optimum reaction temp.
Couleur
colorless
pH
8.1 (39 °F)
Solubilité
water: miscible
Adéquation
suitable for molecular biology
Application(s)
life science and biopharma
sample preparation
Activité étrangère
Endonucleases, none detected (up to 20 U with MWM II-DNA)
Endonucleases, none detected (up to 20U with pBR 322-DNA)
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Compatible ends
Bln I ends are compatible with ends generated by Nhe I, Spe I and Xba I.
Isoschizomers
Bln I is an isoschizomer of Avr II.
Note: The complete 13 site Avr II restriction map of the E.coli genome has been reported.
Methylation sensitivity
The enzyme is not known to be affected by methylation.
Spécificité
CCTAGG
Restriction site: C↓CTAGG
C↓CTAGG
Heat inactivation: No inactivation of Bln I after incubation at 65 °C for 15 minutes.
Qualité
1 μg λDNA is incubated for 16 hours in 50 μl SuRE/Cut Buffer H with an excess of Bln I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5 μg [3H] labeled calf thymus DNA are incubated with 3 μl Bln I for 4 hours at +37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
Typical ligation and recutting assay
Bln I fragments obtained by complete digestion of 1 μg λ × EcoR I DNA ligated for 16 hours at +4°C with 1 U T4 DNA Ligase in 10 μl buffer that contains 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Bln I and EcoR I (yielding the typical pattern of λ × EcoR I × Bln I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
Profil d'ADN
- λ: 2
- φX174: 0
- Ad2: 2
- M13mp7: 0
- M13mp18:0
- pBR322: 0
- pBR328: 0
- pUC18: 0
- SV40: 2
Définition de l'unité
Stockage et stabilité
Remarque sur l'analyse
Bln I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E.coli C 600) embedded in agarose for PFGE analysis, we recommend using 10 U of enzyme/μg DNA and 4 hour incubation.
The buffer in bold is recommended for optimal activity
- A: 25-50%
- B: 50-75%
- H: 100%
- L: 0-10%
- M: 25-50%
Autres remarques
Composants de kit seuls
- Enzyme Solution
- SuRE/Cut Buffer H 10x concentrated
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Articles
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
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