11684302001
Roche
ABTS™ Solution
solution (ready-to-use), suitable for ELISA
Synonyme(s) :
ABTS™ Solution, ABTS
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About This Item
Code UNSPSC :
12352204
Produits recommandés
Forme
solution (ready-to-use)
Niveau de qualité
Utilisation
sufficient for 1,500-3,000 assays (ELISA)
Conditionnement
pkg of 3 × 100 mL
Fabricant/nom de marque
Roche
Technique(s)
ELISA: suitable
Température de stockage
2-8°C
Description générale
ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.
Application
ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
Actions biochimiques/physiologiques
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
Forme physique
Reaction product:
Color: green
Evaluation: photometric (405nm)
Color: green
Evaluation: photometric (405nm)
Remarque sur l'analyse
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Informations légales
ABTS is a trademark of Roche
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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H Matsuda et al.
The Japanese journal of experimental medicine, 54(3), 131-138 (1984-06-01)
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for
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