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11333062910

Roche

Anti-Digoxigenin

from mouse IgG1κ (clone 1.71.256)

Synonyme(s) :

anti-digoxigenin, digoxigenin

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About This Item

Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

clone 1.71.256, monoclonal

Forme

lyophilized

Conditionnement

pkg of 100 μg

Fabricant/nom de marque

Roche

Technique(s)

ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL

Isotype

IgG1κ

Température de stockage

2-8°C

Description générale

Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.

Spécificité

The monoclonal antibody reacts with free and bound digoxigenin.

Application

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Immunofluorescence staining
  • FISH (fluorescent in situ hybridization)

Actions biochimiques/physiologiques

In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.

Caractéristiques et avantages

  • Specific to free and bound digoxigenin
  • The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.

Notes préparatoires

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications
phosphate buffered saline, pH 7.4

Reconstitution

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Stockage et stabilité

The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.

Remarque sur l'analyse

There are no cross reactivities known.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction
<BIG>Lion T and Haas OA</BIG>
Analytical Chemistry, 188, 335-337 (1990)
Magda Rudzka et al.
The Plant cell, 34(6), 2404-2423 (2022-03-17)
Gene regulation ensures that the appropriate genes are expressed at the proper time. Nuclear retention of incompletely spliced or mature mRNAs is emerging as a novel, previously underappreciated layer of posttranscriptional regulation. Studies on this phenomenon indicated that it exerts
Carla Winterling et al.
RNA biology, 11(1), 66-75 (2014-01-21)
A growing body of evidence suggests the non-protein coding human genome is of vital importance for human cell function. Besides small RNAs, the diverse class of long non-coding RNAs (lncRNAs) recently came into focus. However, their relevance for infection, a
Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+-ATPase by N-Hydroxysuccinimidyl Derivatives of Digoxigenin
<BIG>Antolovic R, et al.</BIG>
European Journal of Biochemistry, 227, 61-67 (1995)
Xingqi Chen et al.
BioTechniques, 56(3), 117-118 (2014-03-20)
Current techniques for analyzing chromatin structures are hampered by either poor resolution at the individual cell level or the need for a large number of cells to obtain higher resolution. This is a major problem as it hampers our understanding
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