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11089161001

Roche

Streptavidin-AP conjugate

solution, pkg of 1 mL (1,000U)

Synonyme(s) :

streptavidin

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About This Item

Code UNSPSC :
12352200

Forme

solution

Niveau de qualité

Conditionnement

pkg of 1 mL (1,000U)

Fabricant/nom de marque

Roche

Température de stockage

2-8°C

Description générale

Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.

Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.

Application

Streptavidin-AP has been used to detect biotinylated compounds using:
  • ELISA
  • Immunohistocytochemistry
  • Western blot

Forme physique

Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.

Notes préparatoires

Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.

ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)

Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


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Consulter la Bibliothèque de documents

Ablation of BMP signaling hampers the blastema formation in Poecilia latipinna by dysregulating the extracellular matrix remodeling and cell cycle turnover
Patel S, et al.
Zoology (Jena, Germany), 133, 17-26 (2019)
Daniela Röthlisberger et al.
FEBS letters, 564(3), 340-348 (2004-04-28)
Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound antibody fragment enlarges the hydrophilic part of the mostly hydrophobic membrane protein, thereby increasing the interaction area
Manuela Sauter et al.
iScience, 25(1), 103677-103677 (2022-01-18)
Atherosclerosis is studied in models with dysfunctional lipid homeostasis-predominantly the ApoE-/- mouse. The role of antigen-presenting cells (APCs) for lipid homeostasis is not clear. Using a LacZ reporter mouse, we showed that CD11c+ cells were enriched in aortae of ApoE-/-
Johan L K Van Hove et al.
Molecular genetics and metabolism, 95(4), 201-205 (2008-11-01)
We investigated in a patient with holocarboxylase synthetase deficiency, the relation between the biochemical and genetic factors of the mutant protein with the pharmacokinetic factors of successful biotin treatment. A girl exhibited abnormal skin at birth, and developed in the
Volker Hegenauer et al.
Nature communications, 11(1), 5299-5299 (2020-10-22)
Parasitic plants of the genus Cuscuta penetrate shoots of host plants with haustoria and build a connection to the host vasculature to exhaust water, solutes and carbohydrates. Such infections usually stay unrecognized by the host and lead to harmful host

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