10634425001

Octyl β-D-glucopyranoside

non-ionic

• Synonyme(s) : n-Octyl glucoside, OGP
• Formule empirique (notation de Hill): C₁₄H₂₈O₆
• Numéro CAS: 29836-26-8
• Poids moléculaire : 292.37
• Numéro Beilstein : 84118
• Numéro MDL: MFCD00063288
• Code UNSPSC : 12352200
• ID de substance PubChem : 329748920

Propriétés

• Pureté: 99% (GC)
• Poids mol.: micellar avg mol wt 25,000; 292.4
• Conditionnement: pkg of 10 g
• Fabricant/nom de marque: Roche
• Nombre d'agrégation: 84
• Technique(s): dialysis: suitable
• CMC: 20-25 mM (20-25°C)
• Température de transition: cloud point >100 °C
• Conditions d'expédition: ambient
• Chaîne SMILES: CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
• InChI: 1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
• Clé InChI: HEGSGKPQLMEBJL-RKQHYHRCSA-N

Description

Description générale

1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.

Application

N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.

Détergent non ionique, dialysable, pour la solubilisation et l′isolement des protéines membranaires. Permet d′augmenter la résolution des protéines dans les gels 2D.

Qualité

Purity: 99% (from C), homogeneous (GC)

Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C₁₄H₂₈O₆

Notes préparatoires

Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.

Stockage et stabilité

Store at 15 to 25 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.