10269611001
Roche
Neuraminidase (Sialidase)
from Arthrobacter ureafaciens
Synonyme(s) :
PCR, taq
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
bacterial (Arthrobacter ureafaciens)
Niveau de qualité
Forme
solution
Activité spécifique
~25 units/mg protein
Conditionnement
pkg of 1 U (100 μl)
Fabricant/nom de marque
Roche
pH optimal
5.0-5.5
Température de stockage
2-8°C
Description générale
Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Spécificité
Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.
Application
- cell surface lectin array analysis.
- hemagglutination assays.
- cell adhesion assay.
For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Neuraminidase has been used for the:
- detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells
- release of sialic acid from cells
- antibody-overlay lectin microarray
Propriétés physiques
This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.
Forme physique
Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7
Notes préparatoires
Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
A high-throughput capillary isoelectric focusing immunoassay for fingerprinting protein sialylation.
Lam Raga Anggara Markely et al.
Biotechnology progress, 32(1), 235-241 (2015-11-21)
The serum half-life, biological activity, and solubility of many recombinant glycoproteins depend on their sialylation. Monitoring glycoprotein sialylation during cell culture manufacturing is, therefore, critical to ensure product efficacy and safety. Here a high-throughput method for semi-quantitative fingerprinting of glycoprotein
Sialylation by ??galactoside ??2,6?sialyltransferase and N?glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma
Suzuki O, et al.
International Journal of Oncology, 46(3), 973-980 (2015)
Tomonori Kaifu et al.
The Journal of experimental medicine, 218(12) (2021-11-25)
Dendritic cell immunoreceptor (DCIR) is a C-type lectin receptor with a carbohydrate recognition domain and an immunoreceptor tyrosine-based inhibitory motif. Previously, we showed that Dcir-/- mice spontaneously develop autoimmune enthesitis and sialadenitis, and also develop metabolic bone abnormalities. However, the
Cellular entry of the porcine epidemic diarrhea virus
Li W, et al.
Virus Research, 226, 117-127 (2016)
C K Liu et al.
Journal of virology, 72(6), 4643-4649 (1998-05-30)
The human JC polyomavirus (JCV) is the etiologic agent of the fatal central nervous system (CNS) demyelinating disease progressive multifocal leukoencephalopathy (PML). PML typically occurs in immunosuppressed patients and is the direct result of JCV infection of oligodendrocytes. The initial
Protocoles
Neuraminidase (Sialidase) Protocol
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique