10127655001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

• Synonyme(s) : G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Numéro de classification (Commission des enzymes): 1.1.1.49 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: yeast
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
• Conditionnement: pkg of 1 mL (5 mg/ml)
• Fabricant/nom de marque: Roche
• Concentration: ≥0.1-1.0 % (w/w)
• Technique(s): activity assay: suitable
• Couleur: white to yellowish
• pH optimal: 9.2
• Solubilité: water: miscible
• Adéquation: suitable for UV spectrophotometry and general use
• Numéro d'accès NCBI: CAA93357
• Numéro d'accès UniProt: P11412
• Application(s): life science and biopharma
• Activité étrangère: 6-PGDH <0.0100%; CK <0.00100%; GR <0.0100%; HK <0.0100%; PGI <0.00200%; PGluM <0.0100%
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Informations sur le gène: Saccharomyces cerevisiae ... ZWF1(855480)

Description

Description générale

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP⁺ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP⁺ and NADPH levels the equilibrium shifts to inactive tetramers.

Application

Component of cofactor recycling systems for NADPH.

Actions biochimiques/physiologiques

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP⁺ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Caractéristiques

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI

Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Notes préparatoires

Activator: Mg²⁺ (5 to 10 mM)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash