PMEF-NL-P1

EmbryoMax^® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1

• Synonyme(s) : Mouse Embryonic Fibroblasts
• Code UNSPSC : 41106509
• eCl@ss : 32011203
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Fabricant/nom de marque: Specialty Media; EmbryoMax^®
• Technique(s): cell culture | stem cell: suitable
• Entrée: sample type primary embryotic fibroblasts (PMEFs); sample type induced pluripotent stem cell(s); sample type: mouse embryonic stem cell(s)
• Conditions d'expédition: liquid nitrogen

Description

Description générale

PMEF-NL-P1 are low passage neomycin resistant Primary Mouse Embryo Fibroblasts (PMEFs) isolated from FVB mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

Description de la lignée cellulaire

Primary Mouse Embryo Fibroblasts

Application

Research Category
Stem Cell Research

Conditionnement

1x10⁶ cells

Forme physique

Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.

Stockage et stabilité

After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Informations légales

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable