NE1022
PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31)
liquid, clone SMI-31, Calbiochem®
Synonyme(s) :
Anti-neurofilament antibody
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
SMI-31, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
chicken, Xenopus
Réactivité de l'espèce (prédite par homologie)
mammals
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... NEFH(4744)
Description générale
Mouse monoclonal antibody supplied as purified antibody. Recognizes the ~180-200 kDa phosphorylated neurofilament H protein.
Recognizes the ~180-200 kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein.
This PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31) is validated for use in ELISA, Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of Neurofilament H.
Immunogène
homogenized, hypothalami from Fischer 344 rat brain
Application
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Immunoprecipitation (see comments)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In PBS and Thimerosal
Autres remarques
Raina, A.K., et al. 1999. Neuroreport10, 1355.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Strongly recognizes phosphorylated neurofilament H and, to a lesser extent, phosphorylated neurofilament M. By immunocytochemistry this antibody broadly stains thick and thin axons and some dendrites, such as basket cell dendrites, but not Purkinje cell dendrites. Does not generally stain nerve cell bodies or other cells and tissues except peripheral axons. May also stain neuronal cell bodies in pathological conditions. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures following treatment with agents that induce stress-activated protein kinase. This antibody is reported to co-immunoprecipitate neurofilament-associated kinase (NAK 115) via interaction of the antibody with the tail domain of neurofilament H. Phosphatase treatment of tissue sections or immunoblotting samples abolishes antibody reactivity. Reactivity is unaffected by trypsin treatment of samples. Tissues and cultured cells can be fixed with a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin′s fixative. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the epitope in frozen sections or thick tissue sections fixed in 4% paraformaldehyde and in cultured cells. For staining formalin-fixed, paraffin sections it is recommended that the de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Masamitsu Shimazawa et al.
Journal of neuroscience research, 90(10), 1960-1969 (2012-06-08)
We evaluated time-dependent optic nerve degeneration and the role of endoplasmic reticulum (ER) stress in this process following retinal ganglion cell death in mice. Retinal damage was induced by intravitreal injection of N-methyl-D-aspartate (NMDA). Neurofilament heavy (NFH)- and phosphorylated NFH
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