MABT186

Anti-Exo70 Antibody, clone 70X13F3

clone 70X13F3, from mouse

• Synonyme(s) : Exocyst complex component 7, Exocyst complex component Exo70, rExo70
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 70X13F3, monoclonal
• Espèces réactives: human, rat
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_073182
• Numéro d'accès UniProt: O54922
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EXOC7(23265)

Description

Description générale

The exocyst complex component 70 (Exo70) is a component of the exocyst complex, an octameric structure that is also comprised of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. The exocyst complex facilitates the fusion of secretory vesicles with the plasma membrane and the release of vesicular cargo, which may include neurotransmitters, hormones, and membrane proteins. Previous studies have indicated that Exo70 plays an important role in actin assembly and cell motility.

Immunogène

GST-tagged recombinant protein corresponding to rat Exo70.

Application

Detect Exo70 using this Anti-Exo70 Antibody, clone 70X13F3 validated for use in Western Blotting, ICC.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected Exo70 in 10 µg of human brain tissue lysate.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in Exo70 cells. (Hsu, S.C., et al. (2001). The Journal of Neuroscience. 21(11):3839–3848.)
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hsu, S.C., et al. (2011). The Journal of Neuroscience. 31(10):3522–3535.)

Qualité

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Exo70 in 10 µg of rat brain tissue lysate.

Description de la cible

~75 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat brain tissue lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable