MABT106

Anti-Protein Spindly Antibody, clone 9D10.2

clone 9D10.2, from mouse

• Synonyme(s) : Protein Spindly, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99, Rhabdomyosarcoma antigen MU-RMS-40.4A
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 9D10.2, monoclonal
• Espèces réactives: human, mouse
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgMκ
• Numéro d'accès NCBI: NP_060255
• Numéro d'accès UniProt: Q96EA4
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SPDL1(54908)

Description

Description générale

Protein spindly, otherwise known as the coiled-coil domain-containing protein 99 (CCDC99), is a phosphoprotein that regulates mitosis. It interacts with the Rod/ZW10/Zwilch complex, and is involved in recruiting, docking, and alignment of motor protein dynein at mitotic kinetochores. Spindly may also be involved in checkpoint signaling and microtubule attachment.

Immunogène

GST-tagged recombinant protein corresponding to human Protein Spindly.

Application

Detect Protein Spindly using this Anti-Protein Spindly Antibody, clone 9D10.2 validated for use in ICC, Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Protein Spindly in HeLa and NIH/3T3.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualité

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Protein Spindly in 10 µg of HEK293 cell lysate.

Description de la cible

~70 kDa observed. This protein is known to exist in two isoforms at 70 kDa and 56 kDa An uncharacterized band may be observed at ~38 kDa in some cell lysates.

Forme physique

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HEK293 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable