MABS279

Anti-GPR30, clone 2F2 Antibody

clone 2F2, from mouse

• Synonyme(s) : G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, FEG-1, G-protein coupled receptor 30, GPCR-BR, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, LYGPR
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2F2, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001035055
• Numéro d'accès UniProt: Q99527
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GPER1(2852)

Description

Description générale

GPR30, also known as G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1 (FEG-1), G-protein coupled receptor 30, GPCR-BR, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor (LYGPR), or Membrane estrogen receptor (mER), and encoded by the gene GPER/CEPR/CMKRL2/DRY12/GPR30,is the common receptor for estrogen. GPR30 binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol (3,4,5)-trisphosphate in the nucleus, and therefore GPR30 is part of the rapid non-genomic signaling events widely observed when cells interact with estrogen. GPR30 is a cell membrane bound protein found in the Golgi apparatus, endoplasmic reticulum and plasma membrane. GPR30 is ubiquitously expressed, but is most abundant in placenta. In brain regions, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human GPR30.

Application

Detect GPR30 using this Anti-GPR30 antibody, clone 2F2 validated for use in IHC & IP.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected GPR30 in HEK293 cell lysate (Filardo, E., et al. (2007). Endocrinology. 148(7):3236-3245.).

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Qualité

Evaluated by Immunohistochemistry in human placenta tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GPR30 in human placenta tissues.

Description de la cible

42 kDa calculated

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable