MABS2273

Anti-CD200/HPi1 Antibody, clone HIC0-4F9

• Synonyme(s) : OX-2 membrane glycoprotein
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: HIC0-4F9, monoclonal
• Poids mol.: calculated mol wt 31.26 kDa; observed mol wt ~N/A kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_001004196
• Numéro d'accès UniProt: P41217
• Température de stockage: 2-8°C
• Informations sur le gène: human ... CD200(4345)

Description

Spécificité

Clone HIC0-4F9 is a mouse monoclonal antibody that detects CD200/HPi1 protein.

Immunogène

Human pancreatic enriched islet cells.

Application

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Tested Applications

Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications in fresh-frozen normal pancreas tissues (Courtesy of Craig Dorrell, Ph.D., Oregon Health & Science University, Portland, Oregon USA).

Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications (Dorrell, C., et al. (2008). Stem Cell Res. 1(3):183-94).

Flow Cytometry Analysis: A representative lot detected CD200/HPi1 in Flow Cytometry applications (van der Heide, V., et al. (2022). Cell Rep. 38(11):110508).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

OX-2 membrane glycoprotein (UniProt: P41217; also known as CD200) is encoded by the CD200 (also known as MOX1, MOX2, My033) gene (Gene ID: 4345) in human. CD200 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-232), a transmembrane domain (aa 233-359), and a cytoplasmic domain (aa 260-278). It also contains one V-type Ig-like domain (aa 31-141) and one C2-type Ig-like domain (aa 142-232). It is widely expressed on a broad range of cells including lymphoid cells, neurons, and endothelium. It interacts with CD200R, an immunoglobulin superfamily inhibitory receptor that is expressed primarily on myeloid/monocyte lineage cells and has a suppressive effect on T cell-mediated immune response. CD200 also serves as a pani-slet marker of pancreatic endocrine cells, including insulin-expressing β- cells, but does not recognize exocrine cells. Its levels are reported to be elevated in pancreatic β cells following SARS-CoV-2 infection and this infection is strictly dependent on ACE2 receptor. Three isoforms of CD200 have been described that are produced by alternative splicing. (Ref.: van der Heide, V., et al. (2022). Cell Rep. 38(11); 110508; Dorfman, DM., and Shahsafaei, A. (2010). Am. J. Clin. Pathol. 134(5); 726-733; Dorrell, C., et al. (2008). Stem Cell Res. 1(3); 183-194).

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable