MABN717

Anti-WNT10B Antibody, clone 5A7

ascites fluid, clone 5A7, from mouse

• Synonyme(s) : Protein Wnt-10b, Protein Wnt-12
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 5A7, monoclonal
• Espèces réactives: human
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_003385
• Numéro d'accès UniProt: O00744
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... WNT10B(7480)

Description

Description générale

Wingless-type MMTV integration site family member 10B (WNT10B), also known as Protein Wnt-12 (WNT12), consists of structurally related secreted signaling proteins implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is detected in most adult tissues, with the highest levels found in heart and skeletal muscle, and lower levels found in brain. WNT10B may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. Defects in WNT10B are linked to split-hand/foot malformation type 6 (SHFM6).

Immunogène

Purified recombinant fragment of human WNT10B expressed in E. coli.

Application

Anti-WNT10B Antibody, clone 5A7 is a highly specific mouse monoclonal antibody, that targets Wnt & has been tested in IHC, western blotting & Immunofluorescence.

Immunohistochemistry Analysis: A 1:500-1,000 dilution from a representative lot detected WNT10B in human kidney and liver tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT10B in PANC-1 cells.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WNT10B in 10 µg of HeLa cell lysate.

Description de la cible

~47 kDa observed. Uncharacterized bands may appear in some lysate(s).

Forme physique

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
HeLa cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable