MABN390

Anti-Sigma IR Antibody, clone ICR-SIG1R-A

clone ICR-SIG1R-A, from rat

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: ICR-SIG1R-A, monoclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (immunogen homology)
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2c
• Numéro d'accès NCBI: NP_005857
• Numéro d'accès UniProt: Q99720
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SIGMAR1(10280)

Description

Description générale

Sigma1R, or Sigma non-opioid intracellular receptor 1, is part of the ERG2 family that forms a ternary complex with ANK2 and ITPR3. Sigma1R is involved in lipid transport from the endoplasmic reticulum, BDNF and EGF signaling, potassium ion channel regulation and other cell functions including apoptosis, survival and proliferation. It was initially discovered through its binding to various psychoactive drugs, and has been studied in memory, learning and mood alteration processes. Sigma1R is often found in the nuclear membrane, synapses and endoplasmic reticulum of cells within liver, brain, kidney and thymus tissues.

Immunogène

Linear peptide corresponding to human Sigma IR.

Application

Detect the Sigma IR protein using this Anti-Sigma IR, clone ICR-SIG1R-A validated for use in wb, IP, ICC, Flow & IHC.

Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in certain cancer cell lysates.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in tumor cells.
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Qualité

Evaluated by Western Blotting in colo-587 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sigma IR in 10 µg of colo-587 cell lysate.

Description de la cible

~25 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing at ~25 kDa, ~23 kDa, ~21.5 kDa, ~12 kDa and ~21 kDa

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Colo-587 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable