MABF536

Anti-CD127 (IL-7Ra) Antibody (mouse), Biotin, clone A7R34

clone A7R34, 0.5 mg/mL, from rat

• Synonyme(s) : Interleukin-17 receptor A, IL-17 receptor A, IL-17RA, CD217
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Conjugué: biotin conjugate
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: A7R34, monoclonal
• Espèces réactives: mouse
• Conditionnement: antibody small pack of 25 μg
• Concentration: 0.5 mg/mL
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG2aκ
• Numéro d'accès UniProt: Q60943
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Il7R(16197)

Description

Description générale

The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).

This rat monoclonal antibody Anti-CD127 (IL-7Ra) (mouse), Biotin, clone A7R34 validated for use in IP, Flow Cytometry & IHC for the detection of CD127 (IL-7Ra).

Qualité

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD127 (IL-7Ra) in one million C57Bl/6 splenocytes.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable