MABF3175

Anti-IL-5 Antibody, clone 4A1.6.15

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4A1.6.15, monoclonal
• Poids mol.: calculated mol wt 15.24 kDa; observed mol wt ~226 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG2aκ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_000870.1?
• Numéro d'accès UniProt: P05113
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... IL5(3567)

Description

Description générale

Interleukin-5 (UniProt: P05113; also known as IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF) is encoded by the IL5 gene (Gene ID: 3567) in human. IL-5 is a disulfide-linked homodimeric secreted protein that is synthesized and secreted mainly by the activated CD4+ helper T cells of the Th2 subset, mast cells, and eosinophils. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein (aa 20-134). It serves as a powerful proinflammatory cytokine that is responsible for maturation, proliferation, activation, and migration of eosinophils. It is also a key factor in the upregulation of myeloid progenitors in the bone marrow after airway allergen challenge, and for trafficking from the marrow to the airways in upper or lower airways inflammation. Its biological effects are mediated via selective interaction with IL-5 receptor (IL-5R), which is composed of an a subunit (IL-5Ra) and a non-specific βc heterodimer. As a homodimer it binds to IL-5Ra expressed on eosinophil surface and recruits the βc dimer and induces the assembly of the IL-5/IL-5Ra/βc ternary complex. In the absence of IL-5, IL-5Ra is complexed with the intracellular JAK2, whereas the βc subunit is associated with JAK1. (Ref.: Pelaia, C., et al. (2019). Front. Physiol. 10; 1514).

Spécificité

Clone 4A1.6.15 is a mouse monoclonal antibody that detects human Interleukin-5 (IL-5).

Immunogène

Full-length recombinant human Interleukin-5 (without the signal peptide).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-5.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-5.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-5 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-5, clone 4A1.6.15, Cat. No. MABF3175, is a mouse monoclonal antibody that detects human Interleukin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable