MABF2073

Anti-Rabies Virus CVS-11 Antibody, clone 509-6

clone 509-6, from mouse

• Synonyme(s) : Glycoprotein, Glycoprotein Rabies virus, Rabies virus strain CVS-11
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 509-6, monoclonal
• Espèces réactives: rabies virus
• Réactivité de l'espèce (prédite par homologie): all
• Conditionnement: antibody small pack of 25 μg
• Technique(s): ELISA: suitable; flow cytometry: suitable; neutralization: suitable
• Isotype: IgG2aκ
• Numéro d'accès UniProt: O92284
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Glycoprotein (UniProt: O92284) is encoded by the G gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 509-6 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa 231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672 4678).

Spécificité

Clone 509-6 detected glycoprotein from Rabies virus, strain CVS-11 in L929 cells expressing the rabies protein. It did not detect glycoprotein in wild type L929 cells.

Immunogène

Beta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV).

Application

Anti-Rabies Virus, clone 509-6, Cat. No. MABF2073, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus, strain CVS-11 and has been tested for use in ELISA, Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), and Neutralizing.

Neutralizing Analysis: A representative lot neutralized Rabies Virus (Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5; Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

ELISA Analysis: A representative lot detected Rabies Virus in ELISA applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Rabies Virus in Fluorescence Activated Cell Sorting (FACS) applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

Research Category
Inflammation & Immunology

Qualité

Evaluated by Flow Cytometry in L929 cells expressing the rabies protein and in L929 (WT) cells.

Flow Cytometry Analysis: 1 µg of this antibody detected glycoprotein of Rabies virus in one million L929 cells expressing the rabies protein.

Description de la cible

58.86 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable