MABE2032

Anti-MLK1/MRTF-A Antibody, clone 1A11

• Synonyme(s) : MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein, Myocardin-related transcription factor A
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1A11, monoclonal
• Poids mol.: calculated mol wt 98.92 kDa; observed mol wt ~140 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100
• Technique(s): immunofluorescence: suitable; western blot: suitable
• Isotype: IgG2bκ
• Séquence de l'épitope: N-terminal
• Numéro d'accès Protein ID: NP_001269589
• Numéro d'accès UniProt: Q969V6
• Température de stockage: 2-8°C
• Informations sur le gène: human ... MRTFA(57591)

Description

Spécificité

Clone 1A11 is a mouse monoclonal antibody that detects Myocardin-related transcription factor A (MLK1/MRTF-A). It targets an epitope within the N-terminal region.

Immunogène

GST-tagged recombinant fragment corresponding to 260 amino acids from the N-terminal region of human Myocardin-related transcription factor A (MLK1/MRTF-A).

Application

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MLK1/MRTF-A in Ramos cell lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected MLK1/MRTF-A in Immunofluorescence application (Busche, S., et al. (2010). J Cell Sci. 123(Pt16):2803-9).

Western Blotting Analysis: A representative lot detected MLK1/MRTF-A in Western Blotting application (Weissbach, J., et al. (2016). Mol Cell Biol. 36(10):1526-39).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Myocardin-related transcription factor A (UniProt: Q969V6; also known as MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein) is encoded by the MRTFA (also known as KIAA1438, MAL, MLK1) gene (Gene ID: 57591) in human. MRTF-A is a ubiquitously expressed transcription coactivator that associates with serum response factor (SRF) transcription factor to control expression of genes that regulate cytoskeleton during development, morphogenesis, and cell migration. The MRTFA-SRF complex activity responds to Rho GTPase-induced changes in cellular G-actin concentration, and thereby couples cytoskeletal gene expression to cytoskeletal dynamics. It is reported to bind to G-actin via its RPEL repeats. It is detected in cytoplasm as well as in the nucleus and its subcellular location is tightly regulated by actin. High levels of G-actin in the nucleus, observed during serum deprivation, result in low levels of nuclear MRTF-A, while reduced levels of nuclear G-actin result in accumulation of MRTF-A in the nucleus. G-actin-binding in the cytoplasm inhibits its nuclear import by masking the nuclear localization signal (NLS). In contrast, binding to nuclear G-actin promotes its nuclear export to the cytoplasm. Phosphorylation of serine 6 by ERK inhibits the binding of G-actin, unmasking the NLS and promoting nuclear import. Its N-terminal region is required for its nuclear localization and the C-terminal region mediates its transcriptional activity. (Ref.: Weissbach, J., et al. (2016). Mol. Cell. Biol. 36(10); 1526-1539; Busche, S., et al. (2010). J. Cell Sci. 123(16); 2803-2809).

Forme physique

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable