MABE2005
Anti-RNF4 Antibody, clone 11-3.1
Synonyme(s) :
E3 ubiquitin-protein ligase RNF4, Protein SNURF, RING finger protein 4, Small nuclear ring finger protein
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
11-3.1, monoclonal
Poids mol.
calculated mol wt 21.32 kDa
observed mol wt ~32 kDa
Produit purifié par
using protein G
Espèces réactives
human
Conditionnement
antibody small pack of 100
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG2bκ
Séquence de l'épitope
Unknown
Numéro d'accès Protein ID
Numéro d'accès UniProt
Température de stockage
2-8°C
Informations sur le gène
human ... RNF4(6047)
Spécificité
Clone 11-3.1 is a mouse monoclonal antibody that specifically detects recombinant human RNF4 protein.
Immunogène
GST-tagged, recombinant human RNF4 protein.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Description de la cible
E3 ubiquitin-protein ligase RNF4 (UniProt: P78317; also known as RING finger protein 4, EC:2.3.2.27, Small nuclear ring finger protein, Protein SNURF) is encoded by the RNF4 (also known as SNURF) gene (Gene ID: 6047) in human. RNF4 gene encodes ubiquitin E3 ligase, an enzyme widely expressed in many tissues. RNF4 is mapped to human chromosome 4p16.3. The protein contains a RING-type zinc finger domain and around four small ubiquitin-related modifier (SUMO)-interaction motifs. The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates and the SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate. RNF4 is predominantly localized in the nucleus but can also be found in the cytoplasm. This dual localization allows it to function in multiple cellular compartments, dealing with SUMOylated substrates wherever they are located. The movement between these compartments can be regulated by specific signals or stress conditions. RNF4 is broadly expressed across various tissues, with notable levels in tissues that have high cellular turnover or are particularly responsive to stress, such as in immune cells and testis. It is involved in DNA repair, in transcription and chromatin regulation. It is also associated with the process of hematopoiesis. RNF4 is implicated in acute promyelocytic leukemia, acute myeloid leukemia, Fanconi anemia and aplastic anemia. Its role in maintaining protein homeostasis and responding to cellular stress makes it a key player in health and disease, with its dysfunction being linked to cancer, neurodegeneration, and developmental issues. Understanding the mechanisms regulating RNF4 activity and localization can provide insights into therapeutic strategies for these diseases. (Ref.: Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8. (PMID: 24695317).
Forme physique
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Stockage et stabilité
Recommended storage: +2°C to +8°C.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique