MABC541

Anti-Cullin-3 Antibody, clone 3A8.1

clone 3A8.1, from mouse

• Synonyme(s) : Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Transient receptor potential protein 12, TRP12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, VRL-2, Vanilloid receptor-rel
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3A8.1, monoclonal
• Espèces réactives: monkey, mouse
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_003581
• Numéro d'accès UniProt: Q13618
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CUL3(8452)

Description

Description générale

Cullin-3 (UniProt Q13618) is encoded by the CUL3 (also known as PHA2E, KIAA0617) gene (Gene ID 8452) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Cullin-3 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). Cullin-3-based CRLs rely on Cullin-3 interaction with bric-a-brac/tramtrack/broad-complex (BTB) domain-containing proteins for targets recruitment.

Immunogène

Epitope: C-terminal

GST-tagged recombinant protein corresponding to the C-terminal of human Cullin-3.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Cullin-3 Antibody, clone 3A8.1 is validated for use in Western Blotting for the detection of Cullin-3.

Qualité

Evaluated by Western Blotting in mouse neuroblastoma Neuro-2a (N2a) cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-3 in 10 µg of mouse neuroblastoma Neuro-2a (N2a) cell lysate.

Description de la cible

~88 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable