MABC34

Anti-Beclin-1 Antibody, clone 9A1.1

clone 9A1.1, from mouse

• Synonyme(s) : Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 9A1.1, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_003757
• Numéro d'accès UniProt: Q14457
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... BECN1(8678)

Description

Description générale

The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson′s and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).

Immunogène

GST-tagged recombinant protein corresponding to human Beclin-1.

Application

Anti-Beclin-1 Antibody, clone 9A1.1 detects level of Beclin-1 & has been published & validated for use in Western Blotting, IHC(P), Flow Cytometry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Beclin-1 in human colorectal adenocarcinoma tissue.

Flow Cytometry Analysis: 1 µg from a representative lot detected Beclin-1 in HeLa cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Qualité

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Beclin-1 in 10 µg of Raji cell lysate.

Description de la cible

~54 kDa kDa observed

Liaison

Replaces: MABN16

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Raji cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.