MAB3100-C
Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free)
clone 8A11.2, from mouse
Synonyme(s) :
Gap junction gamma-1 protein, Connexin-45, Gap junction alpha-7 protein, Connexin45, Connexin 45
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
8A11.2, monoclonal
Espèces réactives
rabbit, rat, human, guinea pig
Technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GJC1(10052)
Description générale
Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.
Immunogène
KLH-conjugated linear peptide corresponding to Human Connexin 45.
Application
Immunocytochemistry Analysis: A representative lot detected Connexin 45 in transfected rat C6 cells.
Immunocytochemistry Analysis: A representative lot detected Connexin 45 in Rat aorta endothelial cells and transfected HeLa cells (Sorensen, C.M., et al. (2008) Am J Physiol Regul Integr Comp Physiol. 295:R840–R847).
Immunofluorescence Analysis: A representative lot detected Connexin 45 in HeLa cells and human cardiomyocytes (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).
Immunofluorescence Analysis: A representative lot detected Connexin 45 in normal adult rat bladder (Ikeda Y., et al. (2007) Am J Physiol Renal Physiol. 293(4):F1018–F1025).
Western Blotting Analysis: A representative lot detected Connexin 45 in HeLa and human cardiomyocyte lysate (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).
Western Blotting Analysis: A representative lot detected Connexin 45 in guinea pig atrial and SA node tissue (Jones S.A., et al. (2004) The Journal of Physiology, 560:429-437).
Western Blotting Analysis: A representative lot detected Connexin 45 in WT and transfected HeLa cell lysates (Elenes S., et al. (2001) Biophys J. 81(3):1406-18).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in neural tube of 12-day-old embryo and bone marrow spaces and blood vessels after 4 days of orthodontic treatment in periodontium (Gluhak-Heinrich, J., et al. (2006) Cell Commun Adhes. 13(1-2):115–125).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in endothelial and subintimal smooth muscle gap junction plaques in the rabbit iliac artery (Chaytor, A.T., et al. (2005) British Journal of Pharmacology. 144:108–114).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in rabbit middle cerebral artery (Ujiie H., et al. (2003) Stroke. 34(2):544-50).
Immunocytochemistry Analysis: A representative lot detected Connexin 45 in Rat aorta endothelial cells and transfected HeLa cells (Sorensen, C.M., et al. (2008) Am J Physiol Regul Integr Comp Physiol. 295:R840–R847).
Immunofluorescence Analysis: A representative lot detected Connexin 45 in HeLa cells and human cardiomyocytes (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).
Immunofluorescence Analysis: A representative lot detected Connexin 45 in normal adult rat bladder (Ikeda Y., et al. (2007) Am J Physiol Renal Physiol. 293(4):F1018–F1025).
Western Blotting Analysis: A representative lot detected Connexin 45 in HeLa and human cardiomyocyte lysate (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).
Western Blotting Analysis: A representative lot detected Connexin 45 in guinea pig atrial and SA node tissue (Jones S.A., et al. (2004) The Journal of Physiology, 560:429-437).
Western Blotting Analysis: A representative lot detected Connexin 45 in WT and transfected HeLa cell lysates (Elenes S., et al. (2001) Biophys J. 81(3):1406-18).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in neural tube of 12-day-old embryo and bone marrow spaces and blood vessels after 4 days of orthodontic treatment in periodontium (Gluhak-Heinrich, J., et al. (2006) Cell Commun Adhes. 13(1-2):115–125).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in endothelial and subintimal smooth muscle gap junction plaques in the rabbit iliac artery (Chaytor, A.T., et al. (2005) British Journal of Pharmacology. 144:108–114).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in rabbit middle cerebral artery (Ujiie H., et al. (2003) Stroke. 34(2):544-50).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free) is validated for use in Western Blotting and Immunocytochemistry and Immunofluorescence and Immunohistochemistry for the detection of Connexin 45, near CT, cytoplasmic.
Qualité
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Connexin 45 in 10 µg of U2OS cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Connexin 45 in 10 µg of U2OS cell lysate.
Description de la cible
~45 kDa observed.
Additional uncharacterized bands may be visible in some lysates.
Additional uncharacterized bands may be visible in some lysates.
Liaison
Replaces: MABC3100
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Mariana Zancan et al.
Histology and histopathology, 35(4), 395-403 (2019-09-10)
The posterodorsal medial amygdala (MePD) has an adapted synaptic organization that dynamically modulates reproduction and other social behaviors in rats. Discrete gap junctions between glial cells were previously reported in the MePD neuropil. Connexins (Cx) are components of gap junctions
Ageing-related changes of connexins and conduction within the sinoatrial node.
Jones, Sandra A, et al.
The Journal of Physiology, 560, 429-437 (2004)
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