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MAB3083

Sigma-Aldrich

Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1

ascites fluid, clone P1, Chemicon®

Synonyme(s) :

Anti-CALPAIN4, Anti-CANP, Anti-CANPS, Anti-CAPN4, Anti-CDPS, Anti-CSS1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

P1, monoclonal

Espèces réactives

human, bovine

Ne doit pas réagir avec

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NM_001003962.1
NM_001749.2

Numéro d'accès UniProt

P04632

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CAPNS1(826)

Description générale

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

Spécificité

Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).

Immunogène

Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)

Application

Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

D Bano et al.
Cell death and differentiation, 17(1), 119-133 (2009-08-29)
Cell death requires coordinated intracellular signalling before disassembly of cell architecture by degradative enzymes. Although the death signalling cascades that involve the mitochondria, the ER and the plasma membrane have been extensively characterized, only a handful of studies have examined
Estrogen administration attenuates immobilization-induced skeletal muscle atrophy in male rats.
Sugiura, T; Ito, N; Goto, K; Naito, H; Yoshioka, T; Powers, SK
The Journal of Physiological Sciences : Jps null
R L Mellgren et al.
Biochimica et biophysica acta, 954(2), 154-160 (1988-05-18)
Calpains (EC 3.4.22.17) are nonlysosomal intracellular proteinases which require calcium ion for activity. The calpains are heterodimers composed of a large catalytic subunit and a small subunit which may have a regulatory function during the catalytic cycle. However, whether calpains
Critical role for cathepsin B in mediating caspase-1-dependent interleukin-18 maturation and caspase-1-independent necrosis triggered by the microbial toxin nigericin.
Hentze, H; Lin, XY; Choi, MS; Porter, AG
Cell Death and Differentiation null
A radioimmunologic technique for assessing calpain activation in cells.
R L Mellgren
Methods in molecular biology (Clifton, N.J.), 144, 161-169 (2000-05-20)
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